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mouse anti human total eif2α  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc mouse anti human total eif2α
    A) Western blot for total <t>eIF2α</t> and phosphorylated eIF2α levels from in vitro kinase assays. eIF2α only lane: kinase assay with only eIF2α substrate present. GCN2 only lane: kinase assay with GCN2 Kinase/HRSL eluted from pulldown only. No eIF2α substrate present. Naïve only lane: cell lysate from naïve cells (cells were not transfected with plasmid bearing GCN2 Kinase/HRSL) underwent a mock pull-down and elution with flag peptide similar to GCN2 Kinase/HRSL pulldown. Performed kinase assay with naïve cell eluate only (No eIF2α or GCN2 Kinase/HRSL eluate present) (B) replicate. (C) Replicate with western blot for GCN2 Kinase/HRSL using anti-flag antibody.
    Mouse Anti Human Total Eif2α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 2900 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+total+eif2%CE%B1/bio_rxiv__2024__04__24__591037-243-29-33?v=Cell+Signaling+Technology+Inc
    Average 97 stars, based on 2900 article reviews
    mouse anti human total eif2α - by Bioz Stars, 2026-08
    97/100 stars

    Images

    1) Product Images from "Cryo-EM Structure of HRSL Domain Reveals Activating Crossed Helices at the Core of GCN2"

    Article Title: Cryo-EM Structure of HRSL Domain Reveals Activating Crossed Helices at the Core of GCN2

    Journal: bioRxiv

    doi: 10.1101/2024.04.24.591037

    A) Western blot for total eIF2α and phosphorylated eIF2α levels from in vitro kinase assays. eIF2α only lane: kinase assay with only eIF2α substrate present. GCN2 only lane: kinase assay with GCN2 Kinase/HRSL eluted from pulldown only. No eIF2α substrate present. Naïve only lane: cell lysate from naïve cells (cells were not transfected with plasmid bearing GCN2 Kinase/HRSL) underwent a mock pull-down and elution with flag peptide similar to GCN2 Kinase/HRSL pulldown. Performed kinase assay with naïve cell eluate only (No eIF2α or GCN2 Kinase/HRSL eluate present) (B) replicate. (C) Replicate with western blot for GCN2 Kinase/HRSL using anti-flag antibody.
    Figure Legend Snippet: A) Western blot for total eIF2α and phosphorylated eIF2α levels from in vitro kinase assays. eIF2α only lane: kinase assay with only eIF2α substrate present. GCN2 only lane: kinase assay with GCN2 Kinase/HRSL eluted from pulldown only. No eIF2α substrate present. Naïve only lane: cell lysate from naïve cells (cells were not transfected with plasmid bearing GCN2 Kinase/HRSL) underwent a mock pull-down and elution with flag peptide similar to GCN2 Kinase/HRSL pulldown. Performed kinase assay with naïve cell eluate only (No eIF2α or GCN2 Kinase/HRSL eluate present) (B) replicate. (C) Replicate with western blot for GCN2 Kinase/HRSL using anti-flag antibody.

    Techniques Used: Western Blot, In Vitro, Kinase Assay, Transfection, Plasmid Preparation

    A) Western blot for eIF2α phosphorylation from in vitro kinase assay with GCN2 Kinase/HRSL +/- RNase A treatment (350 nM final concentration). Western blot for flag-tagged GCN2 Kinase/HRSL controls for equal loading. (B-C) Biological replicates related to A . D) Results for three replicates (A-C) quantified and normalized to total eIF2α.
    Figure Legend Snippet: A) Western blot for eIF2α phosphorylation from in vitro kinase assay with GCN2 Kinase/HRSL +/- RNase A treatment (350 nM final concentration). Western blot for flag-tagged GCN2 Kinase/HRSL controls for equal loading. (B-C) Biological replicates related to A . D) Results for three replicates (A-C) quantified and normalized to total eIF2α.

    Techniques Used: Western Blot, Phospho-proteomics, In Vitro, Kinase Assay, Concentration Assay



    Similar Products

    mouse anti human total eif2α  (Cell Signaling Technology Inc)
    97
    Cell Signaling Technology Inc mouse anti human total eif2α
    A) Western blot for total <t>eIF2α</t> and phosphorylated eIF2α levels from in vitro kinase assays. eIF2α only lane: kinase assay with only eIF2α substrate present. GCN2 only lane: kinase assay with GCN2 Kinase/HRSL eluted from pulldown only. No eIF2α substrate present. Naïve only lane: cell lysate from naïve cells (cells were not transfected with plasmid bearing GCN2 Kinase/HRSL) underwent a mock pull-down and elution with flag peptide similar to GCN2 Kinase/HRSL pulldown. Performed kinase assay with naïve cell eluate only (No eIF2α or GCN2 Kinase/HRSL eluate present) (B) replicate. (C) Replicate with western blot for GCN2 Kinase/HRSL using anti-flag antibody.
    Mouse Anti Human Total Eif2α, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+total+eif2%CE%B1/bio_rxiv__2024__04__24__591037-243-29-33?v=Cell+Signaling+Technology+Inc
    Average 97 stars, based on 1 article reviews
    mouse anti human total eif2α - by Bioz Stars, 2026-08
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Western blot for total eIF2α and phosphorylated eIF2α levels from in vitro kinase assays. eIF2α only lane: kinase assay with only eIF2α substrate present. GCN2 only lane: kinase assay with GCN2 Kinase/HRSL eluted from pulldown only. No eIF2α substrate present. Naïve only lane: cell lysate from naïve cells (cells were not transfected with plasmid bearing GCN2 Kinase/HRSL) underwent a mock pull-down and elution with flag peptide similar to GCN2 Kinase/HRSL pulldown. Performed kinase assay with naïve cell eluate only (No eIF2α or GCN2 Kinase/HRSL eluate present) (B) replicate. (C) Replicate with western blot for GCN2 Kinase/HRSL using anti-flag antibody.

    Journal: bioRxiv

    Article Title: Cryo-EM Structure of HRSL Domain Reveals Activating Crossed Helices at the Core of GCN2

    doi: 10.1101/2024.04.24.591037

    Figure Lengend Snippet: A) Western blot for total eIF2α and phosphorylated eIF2α levels from in vitro kinase assays. eIF2α only lane: kinase assay with only eIF2α substrate present. GCN2 only lane: kinase assay with GCN2 Kinase/HRSL eluted from pulldown only. No eIF2α substrate present. Naïve only lane: cell lysate from naïve cells (cells were not transfected with plasmid bearing GCN2 Kinase/HRSL) underwent a mock pull-down and elution with flag peptide similar to GCN2 Kinase/HRSL pulldown. Performed kinase assay with naïve cell eluate only (No eIF2α or GCN2 Kinase/HRSL eluate present) (B) replicate. (C) Replicate with western blot for GCN2 Kinase/HRSL using anti-flag antibody.

    Article Snippet: Following 15 minutes incubation with blocking buffer [5% (w/v) nonfat dry milk in TRIS-Buffered Saline and Tween-20 (standard TBST)], the membranes were probed with the following primary antibodies: 1) mouse anti-human total eIF2α (Cell Signaling Technology) at 1:1,000, 2) rabbit anti-human eIF2α-phosphoS51 (Abcam) at 1:1,000 3) mouse anti-Flag M2 (Sigma) at 1:2,000.

    Techniques: Western Blot, In Vitro, Kinase Assay, Transfection, Plasmid Preparation

    A) Western blot for eIF2α phosphorylation from in vitro kinase assay with GCN2 Kinase/HRSL +/- RNase A treatment (350 nM final concentration). Western blot for flag-tagged GCN2 Kinase/HRSL controls for equal loading. (B-C) Biological replicates related to A . D) Results for three replicates (A-C) quantified and normalized to total eIF2α.

    Journal: bioRxiv

    Article Title: Cryo-EM Structure of HRSL Domain Reveals Activating Crossed Helices at the Core of GCN2

    doi: 10.1101/2024.04.24.591037

    Figure Lengend Snippet: A) Western blot for eIF2α phosphorylation from in vitro kinase assay with GCN2 Kinase/HRSL +/- RNase A treatment (350 nM final concentration). Western blot for flag-tagged GCN2 Kinase/HRSL controls for equal loading. (B-C) Biological replicates related to A . D) Results for three replicates (A-C) quantified and normalized to total eIF2α.

    Article Snippet: Following 15 minutes incubation with blocking buffer [5% (w/v) nonfat dry milk in TRIS-Buffered Saline and Tween-20 (standard TBST)], the membranes were probed with the following primary antibodies: 1) mouse anti-human total eIF2α (Cell Signaling Technology) at 1:1,000, 2) rabbit anti-human eIF2α-phosphoS51 (Abcam) at 1:1,000 3) mouse anti-Flag M2 (Sigma) at 1:2,000.

    Techniques: Western Blot, Phospho-proteomics, In Vitro, Kinase Assay, Concentration Assay